[{"command":"settings","settings":{"pluralDelimiter":"\u0003","suppressDeprecationErrors":true,"user":{"uid":0,"permissionsHash":"d9587e6f410d2e7f476e3da6cb10a457c78ab82347f962bf83d9020620f901dd"}},"merge":true},{"command":"add_css","data":[{"rel":"stylesheet","media":"all","href":"\/modules\/contrib\/addtocal\/addtocal.css?t2408i"},{"rel":"stylesheet","media":"all","href":"\/themes\/custom\/cest2025\/css\/components\/node.css?t2408i"}]},{"command":"add_js","selector":"body","data":[{"src":"\/core\/assets\/vendor\/jquery\/jquery.min.js?v=3.7.1"},{"src":"\/core\/assets\/vendor\/once\/once.min.js?v=1.0.1"},{"src":"\/core\/misc\/drupalSettingsLoader.js?v=10.5.1"},{"src":"\/core\/misc\/drupal.js?v=10.5.1"},{"src":"\/core\/misc\/drupal.init.js?v=10.5.1"},{"src":"\/modules\/contrib\/addtocal\/addtocal.js?v=10.5.1"},{"src":"\/modules\/contrib\/addtocal\/addtocal-download.js?v=10.5.1"}]},{"command":"openDialog","selector":"#drupal-modal","settings":null,"data":"\n\u003Carticle class=\u0022node node--type-presentation node--promoted node--view-mode-modal\u0022\u003E\n  \n      \u003Cb\u003E\u003Cspan\u003ENickel-induced multimetal uptake in two microalgal species\u003C\/span\u003E\n\u003C\/b\u003E\n  \n      \u003Cdiv\u003E\u003Cb\u003ECEST ID: cest2025_00402\u003C\/b\u003E\u003C\/div\u003E\n  \n        \u003Cdiv class=\u0022mb-3\u0022\u003E\n      \u003Cb\u003ERoom  | Sat 6 Sep 2025 | 17:00 - 18:00 pm\u003C\/b\u003E\n    \u003C\/div\u003E\n  \n          \n    \n  \n      \u003Cdiv class=\u0022mt-10\u0022\u003E\n            \u003Cdiv class=\u0022clearfix text-formatted field field--name-presentation-body field--type-text-long field--label-hidden field__item\u0022\u003EWe investigated the effect of Ni(II) uptake on two microalgal species, Chlorella sorokiniana, and Chlamydomonas reinhardtii, in 1 - 6 ppm Ni(II) enriched tris-acetate-phosphate (TAP) growth medium. Even though increased uptake of Ni(II) was observed for both algal species, accompanied by growth suppression at high Ni(II) concentrations, multi-elemental trace analysis revealed a significant, Ni concentration-dependent, uptake of incubation media essential metals as well. For both algal species, Zn uptake concentrations increased by approximately 20 times when going from control, no Ni(II), to cultures incubated with increasing Ni(II) concentrations. Overall, Zn uptake was determined to be approximately 3 orders of magnitude higher than Ni(II) uptake when high concentrations of Ni(II) were present. Similar uptake trends were observed for Cu and Co for both algae species, even though uptake concentrations were much lower than those of Zn. For Chlorella sorokiniana, increased Fe uptake relative to Ni(II) uptake was observed (2 orders of magnitude higher), as was the case for Mn (1 order of magnitude higher). This induced increase in uptake of some of the growth media metals was attributed to their liberation from EDTA in TAP medium, following the addition of Ni(II), which has a higher stability constant (Kf) with EDTA and was added at comparable or higher than them concentrations. Calculated levels of free Ni(II) and free metals in the medium matched the observed metal uptake trends as determined using multielemental inductively coupled plasma mass spectrometry. The lipid unsaturation level and relative \u03c9-3 fatty acids concentration of both microalgal species, based on 1H Nuclear Magnetic Resonance analysis, decreased with increasing Ni(II) concentration. Unsaturation levels for individual lipid classes [monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), and sulfoquinovosyldiacylglycerol (SQDG)] in Chlamydomonas reinhardtii cells were also studied using positive ion mode electrospray mass spectrometry. At the highest Ni(II) concentrations, an overall reduction in unsaturation levels was observed for all 3 lipid classes.\u003C\/div\u003E\n      \u003C\/div\u003E\n  \n  \u003Cdiv class=\u0022mt-5 mb-5\u0022\u003E\n          \u003Cspan\u003E\n          \u003Cb\u003EPresenter:\u003C\/b\u003E\n                      \u003Cp\u003E\n            Prof Nikos Lydakis-Simantiris\n            \u003C\/p\u003E\n                  \u003C\/span\u003E\n      \u003C\/div\u003E\n\n  \u003Cdiv class=\u0022mb-5\u0022\u003E\n          \u003Cdiv class=\u0022field__label\u0022\u003E\n        Authors\n      \u003C\/div\u003E\n              \u003Cp\u003E\n          Nikos Lydakis-Simantiris\n        \u003C\/p\u003E\n              \u003Cp\u003E\n          Georgia Panagou\n        \u003C\/p\u003E\n              \u003Cp\u003E\n          Evangelos Stergiou\n        \u003C\/p\u003E\n              \u003Cp\u003E\n          Apostolos Spyros\n        \u003C\/p\u003E\n              \u003Cp\u003E\n          Spiros Pergantis\n        \u003C\/p\u003E\n            \u003C\/div\u003E\n\n\u003C\/article\u003E\n","dialogOptions":{"width":"700","position":{"my":"right top","at":"right top"},"closeOnEscape":true,"dialogClass":"presentation-dialog","modal":true,"title":"","classes":{"ui-dialog":"presentation-dialog"}}}]